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Determination of alkali-labile phosphoprotein phosphorus from fish plasma using the Tb^3+-tiron complex as a fluorescence probe 总被引:1,自引:0,他引:1
A sensitive method based on the fluorescence quenching effect of the Tb^3+-Tiron complex is proposed for the determination of alkali-labile phosphoprotein phosphorus (ALP) released from fish plasma. The detection limit was 5.4 ng/ml (S/N=2), and the relative standard deviation of the quenching effect (6 replicates) was 4.6%. The results obtained by the proposed method were in good agreement with those obtained by the colorimetric assay. The advantages of the present method are its relatively simple detection procedure, the lack of toxic organic solvents, and high sensitivity. 相似文献
94.
A new resonance scattering method was proposed for the determination of chlorite, basing on the resonance scattering effect of rhodamine dye. In HCl-sodium acetate buffer solution, chlorite oxidizes 1- into 12 and the reaction of 12 and excess 1- results in If. It is respectively combined with rhodaminc dyes, including rhodamine B (RhB), butyl rhodamine B (b-RhB), rhodamine G (RhG) and rhodamine S (RhS), to form association complex particles, which exhibit stronger resonance scattering (RS) effect at 400 nm. The chlorite concentration of ClO2 in the range of 0.00726-0.218 μg/ml, 0.0102-0.292 μg/ml, 0.00726-0.145 μg/ml and 0.0290- 0.174 μg/ml is respectively linear to the RS intensity of association complex particle systems at 400 nm for the RhB, b-RhB, RhG and RhS. The detection limits of the four systems were respectively 0.00436, 0.00652, 0.00580 and 0.01450μg/ml ClO2^-. In the four systems, the RhB system possesses good stability and high sensitivity. It has been applied to the analysis of chlorite in wastewater with satisfactory results. 相似文献
95.
Boris N. Porfiriev 《Environmental management》1996,20(1):25-33
An analysis is presented of the environmental effects of the most serious radiation accident recorded after Chernobyl, which
occurred in the formerly secret town of Tomsk-7 in Siberia, Russia, on 6, April 1993. Fortunately, it appears not to have
become a major industrial crisis or disaster. The causes of the accident are described. It is argued that a mixture of both
objective and subjective prerequisites, including specific human, organizational, and technological factors, were responsible
for the explosion or directly facilitated it. The Tomsk-7 accident's ecological, medical, social, and psychological consequences
are discussed. 相似文献
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Paul Harvey 《Disasters》1998,22(3):200-217
The paper examines the challenge of rehabilitation from complex political emergencies (CPEs) and identifies a strategy that is characterised as a civil society rebuilding approach. It focuses on Somalia and a case study of a CARE project that aims to build the capacity of local NGOs. The paper argues that civil society in CPEs is simultaneously being undermined and contested by warring parties and emerging after state collapse. The scope of the paper is limited to one case study and that case study examines only a single aspect of civil society: national and international NGOs. The paper therefore presents tentative and preliminary results based on limited research. However, in reviewing the literature and presenting a way of approaching the subject, it aims to suggest a starting-point for developing a theoretical framework for such research. The paper finds that international agencies have tended to focus on civil society institutions simply as conduits for aid money and that this has tended to create organisations which lack downward accountability, are dependent on donors and are not addressing the wider roles for civil society envisaged in the approach. Rebuilding civil society does hold out the promise of giving non-military interests a stronger voice and starting a process of changing the aid delivery culture. Achieving these objectives, however, will be a slow and largely indigenous process and there is a need for lowered expectations about what outside assistance can achieve 相似文献
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石墨炉原子吸收光谱法测定海河下游水中痕量镉 总被引:2,自引:0,他引:2
原子吸收光谱法直接测定高盐水中痕量镉时,有很大背景吸收和误差。本文采用络合—萃取技术使共存元素与待测元素分离,既消除了基体干扰,又达到了富集作用,使测定结果准确可靠。 相似文献